Composite

Part:BBa_M1212:Design

Designed by: Lindsey Stevens   Group: UtahState_BE5930_-_S11   (2011-04-25)


Kill Gene with CFP alternative


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2161
    Illegal NheI site found at 2184
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1766
    Illegal AgeI site found at 1836
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2437


Design Notes

This composite is meant to operate with a ROSE 1 composite.


Source

The holin comes from a bacteriophage

References